| IGFBP-5

Insulin-like growth factor-binding protein 5 (IBF-5) is a protein encoded by the IGFBP5 gene.It is expressed by fibroblasts, myoblasts, and osteoblasts, making it the most dominant IGFBP found in bone extracts. IGFBP-5 has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When it binds to the extracellular matrix, IGFBP-5 is protected from protein breakdown and can enhance IGF activity. Diseases associated with IGFBP5 include insulin-like growth factor I and renal osteodystrophy.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	2000	1000	500	250	125	62.5	31.25	0
OD	2.36	1.56	1.04	0.65	0.46	0.34	0.29	0.1
Corrected OD	2.26	1.46	0.94	0.55	0.36	0.24	0.19	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat IGFBP-5 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	94-105	104

 

| Linearity

Samples were spiked with high concentrations of Rat IGFBP-5 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	88-96
	Average (%)	91	93
1 : 4	Range (%)	89-105	87-109
	Average (%)	96	98